speedvac concentrator (Eppendorf AG)

Eppendorf AG is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Eppendorf AG speedvac concentrator
Speedvac Concentrator, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 201853 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eppendorf+speedvac/Eppendorf/pm42119924-68-7-9
Average 99 stars, based on 201853 article reviews

speedvac concentrator - by Bioz Stars, 2026-10

99/100 stars

Images

Extraction:

Article Title: Thermodynamic stability modulates chaperone-mediated disaggregation of α-synuclein fibrils
Article Snippet: 50 μL of extraction buffer (5% formic acid in 50% acetonitrile) was added and samples incubated at 37 °C with constant shaking for 30 min. Supernatants containing the peptides were transferred to clean Lo-Bind Eppendorf tubes. .. Extraction was repeated and the pooled supernatants were concentrated in an Eppendorf Speedvac and re-constituted in 12 μL 2% acetonitrile, 1% TFA containing iRT peptides (Biognosys). ..

Article Title: Emerging Synbiotic Interactions between Vanillin and the Probiotic Bacterium <i>Lacticaseibacillus rhamnosus</i> GG Determined via Physiological and Proteomic Approaches
Article Snippet: The supernatants, containing the peptides, were transferred to clean Lo-Bind Eppendorf tubes. .. This extraction process was repeated, and the pooled supernatants were concentrated (Eppendorf Speedvac) and reconstituted in 12 μL of 2% acetonitrile, 1% TFA, containing iRT peptides (Biognosys). .. The peptides were then centrifuged at 21000 × g for 10 min. For each sample, peptides were loaded onto a 2 cm C18 trap column (Thermo Fisher 164705) connected in-line to a 15 cm C18 reverse-phase analytical column (Thermo EasySpray ES803) using 100% Buffer A (0.1% formic acid in water) at a pressure of 750 bar.

Multiplex sample analysis:

Article Title: Temporal lobe epilepsy is associated with neuroinflammation, extracellular matrix remodeling, and synaptic protein alterations
Article Snippet: After washing the tips twice with 200 μl of 0.1% formic acid, the peptides were eluted into clean Eppendorf Protein LoBind tubes using 40% acetonitrile, 0.1% formic acid. .. The eluted peptides were concentrated in an Eppendorf Speedvac and reconstituted in 50 mM HEPES pH 8.5 for TMT labeling with 16plex tags (Thermo ScientificTM, St. Louis, USA). ..

Article Title: Visceral signaling of post-ingestive malaise directs memory updating in Drosophila
Article Snippet: After washing the tips twice with 200ul of 0.1% formic acid, the peptides were eluted into clean 0.5ml Eppendorf tubes using 40% Acetonitrile, 0.1% formic acid. .. The eluted peptides were concentrated in an Eppendorf Speedvac and re-constituted in 20uL 50mM HEPES (pH8.5) for TMT labelling with 18plex tags (Thermo). ..

Labeling:

Incubation:

Article Title: Global matrisome changes in obese lung are linked to fibroblastic stroma and premature aging.
Article Snippet: After washing, the beads were suspended in 75 μL 100 mM TEAB with 10 ng/μL trypsin (Sigma), sonicated for 30 s in a water bath sonicator and incubated for 18 h at 37 ◦ C, shaking at 1,000 RPM. .. After incubation, supernatant containing peptides was transferred to clean tubes and concentrated in an Eppendorf SpeedVac. .. Finally, samples were reconstituted in 12 μL 2% acetonitrile, 1% TFA containing iRT peptides (Biognosys).

Review